Acetyl-Histone H4-K8 pAb, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
PRS-22-817
- Bilder (7)
| Artikelname: | Acetyl-Histone H4-K8 pAb, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | PRS-22-817 |
| Hersteller Artikelnummer: | 22-817 |
| Alternativnummer: | PRS-22-817-100 |
| Hersteller: | ProSci |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ChIP, IF, IHC, IP, WB |
| Spezies Reaktivität: | Mouse, Rat |
| Immunogen: | A synthetic peptide of human Acetyl-Histone H4-K8 |
| Konjugation: | Unconjugated |
| Alternative Synonym: | H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4, Histone H4H4FA |
| Application Verdünnung: | Optimal dilutions for each application to be determined by the researcher. |
| Anwendungsbeschreibung: | WB: 1:1000 - 1:3000IHC: 1:200 - 1:500IF: 1:500 - 1:1000IP: 1:200 - 1:500CHIP: 1:20 - 1:50CHIP seq: 1:20 - 1:50 |
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Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K8 antibody (22-817) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s. |
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Immunohistochemistry of paraffin-embedded rat ovary using H4K8ac antibody (22-817) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human breast using H4K8ac antibody (22-817) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human gastric cancer using H4K8ac antibody (22-817) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse brain using H4K8ac antibody (22-817) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of HeLa cells using Acetyl-Histone H4-K8 antibody (22-817). Blue: DAPI for nuclear staining. |
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Chromatin immunoprecipitation analysis of extracts of 293 cell line, using H4K8ac antibody (22-817) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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