NAA10 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-22-628
Artikelname: NAA10 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-22-628
Hersteller Artikelnummer: 22-628
Alternativnummer: PRS-22-628-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 161-235 of human NAA10 (NP_003482.1).
Konjugation: Unconjugated
Alternative Synonym: ARD1A, ARD1, DXS707, MGC71248, TE2, NATD, ARD1A, ARD1P, MCOPS1
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 26kDa
NCBI: 8260
UniProt: P41227
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using NAA10 antibody (22-628) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat lung using NAA10 antibody (22-628) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using NAA10 antibody (22-628) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using NAA10 antibody (22-628) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using NAA10 antibody (22-628) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using NAA10 antibody (22-628). Blue: DAPI for nuclear staining.