SOX5 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-22-607
Artikelname: SOX5 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-22-607
Hersteller Artikelnummer: 22-607
Alternativnummer: PRS-22-607-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SOX5 (NP_694534.1).
Konjugation: Unconjugated
Alternative Synonym: SOX5, L-SOX5, L-SOX5B, L-SOX5F, RP11-444D3.1
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 70kDa
NCBI: 6660
UniProt: P35711
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using SOX5 antibody (22-607) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat kidney using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SOX5 antibody (22-607) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using SOX5 antibody (22-607).