PNKP Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
PRS-22-410
- Bilder (7)
| Artikelname: | PNKP Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | PRS-22-410 |
| Hersteller Artikelnummer: | 22-410 |
| Alternativnummer: | PRS-22-410-100 |
| Hersteller: | ProSci |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 60-320 of human PNKP (NP_009185.2). |
| Konjugation: | Unconjugated |
| Alternative Synonym: | PNKP, polynucleotide kinase 3-phosphatase, PNK, polynucleotide kinase 3-prime-phosphatase, MCSZ, EIEE10 |
| Application Verdünnung: | Optimal dilutions for each application to be determined by the researcher. |
| Anwendungsbeschreibung: | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100 |
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Western blot analysis of extracts of various cell lines, using PNKP antibody (22-410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
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Immunohistochemistry of paraffin-embedded rat kidney using PNKP antibody (22-410) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human lung cancer using PNKP antibody (22-410) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human prostate cancer using PNKP antibody (22-410) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse liver using PNKP antibody (22-410) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse heart using PNKP antibody (22-410) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using PNKP antibody (22-410). Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region. |
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