DDX41 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-22-325
Artikelname: DDX41 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-22-325
Hersteller Artikelnummer: 22-325
Alternativnummer: PRS-22-325-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, IP, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DDX41 (NP_057306.2).
Konjugation: Unconjugated
Alternative Synonym: DDX41 Antibody: ABS, ABS, Probable ATP-dependent RNA helicase DDX41, DEAD box protein 41
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 75kDa
NCBI: 51428
UniProt: Q9UJV9
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using DDX41 antibody (22-325) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat kidney using DDX41 antibody (22-325) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using DDX41 antibody (22-325) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using DDX41 antibody (22-325) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using DDX41 antibody (22-325). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug DDX41 antibody (22-325). Western blot was performed from the immunoprecipitate using DDX41 antibody (22-325) at a dilition of 1:1000.