CD98 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-19-804
Artikelname: CD98 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-19-804
Hersteller Artikelnummer: 19-804
Alternativnummer: PRS-19-804-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 360-529 of human CD98 (NP_001013269.1).
Konjugation: Unconjugated
Alternative Synonym: 4F2 cell-surface antigen heavy chain, 4F2hc, 4F2 heavy chain antigen, Lymphocyte activation antigen 4F2 large subunit, Solute carrier family 3 member 2, CD98, SLC3A2, MDU1
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 100kDa
NCBI: 6520
UniProt: P08195
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using CD98 antibody (19-804) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 25s.
Immunohistochemistry of paraffin-embedded human breast cancer using CD98 antibody (19-804) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using CD98 antibody (19-804) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using CD98 antibody (19-804) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CD98 Polyclonal Antibody (19-804) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using CD98 Polyclonal Antibody (19-804) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.