CTNNA1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-19-768
Artikelname: CTNNA1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-19-768
Hersteller Artikelnummer: 19-768
Alternativnummer: PRS-19-768-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC, IP, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 637-906 of human CTNNA1 (NP_001894.2).
Konjugation: Unconjugated
Alternative Synonym: Catenin alpha-1, Alpha E-catenin, Cadherin-associated protein, Renal carcinoma antigen NY-REN-13, CTNNA1
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 115kDa
NCBI: 1495
UniProt: P35221
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using CTNNA1 antibody (19-768) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat kidney using CTNNA1 antibody (19-768) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human vermiform appendix using CTNNA1 antibody (19-768) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using CTNNA1 antibody (19-768) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using CTNNA1 antibody (19-768) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug CTNNA1 antibody (19-768). Western blot was performed from the immunoprecipitate using CTNNA1 antibody (19-768) at a dilition of 1:500.