HSD17B10 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-19-669
Artikelname: HSD17B10 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-19-669
Hersteller Artikelnummer: 19-669
Alternativnummer: PRS-19-669-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human HSD17B10 (NP_004484.1).
Konjugation: Unconjugated
Alternative Synonym: HADH2, ABAD, ERAB, MHBD, HSD17B10, 17b-HSD10, hydroxyacyl-Coenzyme A dehydrogenase, type II, type 10 17b-HSD, AB-binding alcohol dehydrogenase, type 10 17beta-hydroxysteroid dehydrogenase, HADH2, MRPP2, SCHAD, XH98G2
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 26kDa
NCBI: 3028
UniProt: Q99714
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using HSD17B10 antibody (19-669) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using HSD17B10 antibody (19-669) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human tonsil using HSD17B10 antibody (19-669) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using HSD17B10 antibody (19-669) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using HSD17B10 antibody (19-669) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using HSD17B10 Polyclonal Antibody (19-669) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.