HDGF Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-19-606
Artikelname: HDGF Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-19-606
Hersteller Artikelnummer: 19-606
Alternativnummer: PRS-19-606-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HDGF (NP_004485.1).
Konjugation: Unconjugated
Alternative Synonym: Hepatoma-derived growth factor, HDGF, High mobility group protein 1-like 2, HMG-1L2, HDGF, HMG1L2
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 38kDa
NCBI: 3068
UniProt: P51858
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using HDGF antibody (19-606) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using HDGF Antibody (19-606) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using HDGF Antibody (19-606) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using HDGF Antibody (19-606) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using HDGF antibody (19-606) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using HDGF antibody (19-606) at dilution of 1:100. Blue: DAPI for nuclear staining.