DiMethyl-Histone H3-K14 pAb, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-19-559
Artikelname: DiMethyl-Histone H3-K14 pAb, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-19-559
Hersteller Artikelnummer: 19-559
Alternativnummer: PRS-19-559-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ChIP, IF, IHC, IP, WB
Spezies Reaktivität: Mouse, Rat
Immunogen: A synthetic peptide of human DiMethyl-Histone H3-K14
Konjugation: Unconjugated
Alternative Synonym: H3F3A, H3t, H3.4, H3/g, H3FT
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 16kDa
NCBI: 8290
UniProt: Q16695
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100
Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K14 antibody (19-559).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K14 antibody (19-559) at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat liver using DiMethyl-Histone H3-K14 antibody (19-559) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human rectal cancer using DiMethyl-Histone H3-K14 antibody (19-559) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of C6 cells using DiMethyl-Histone H3-K14 pAb (19-559) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using DiMethyl-Histone H3-K14 pAb (19-559) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using DiMethyl-Histone H3-K14 pAb (19-559) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.