UBE2L3 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-19-297
Artikelname: UBE2L3 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-19-297
Hersteller Artikelnummer: 19-297
Alternativnummer: PRS-19-297-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 73-212 of human UBE2L3 (NP_001243284.1).
Konjugation: Unconjugated
Alternative Synonym: UBE2L3, E2-F1, L-UBC, UBCH7, UbcM4
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 18kDa
NCBI: 7332
UniProt: P68036
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:1000 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using UBE2L3 antibody (19-297) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat heart using UBE2L3 antibody (19-297) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using UBE2L3 antibody (19-297) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using UBE2L3 antibody (19-297) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using UBE2L3 Polyclonal Antibody (19-297) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using UBE2L3 Polyclonal Antibody (19-297) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.