COX6A1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-19-164
Artikelname: COX6A1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-19-164
Hersteller Artikelnummer: 19-164
Alternativnummer: PRS-19-164-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-109 of human COX6A1 (NP_004364.2).
Konjugation: Unconjugated
Alternative Synonym: Cytochrome c oxidase subunit 6A1, mitochondrial, Cytochrome c oxidase polypeptide VIa-liver, Cytochrome c oxidase subunit VIA-liver, COX VIa-L, COX6A1, COX6AL
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 12kDa
NCBI: 1337
UniProt: P12074
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:200 - 1:2000IHC: 1:20 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using COX6A1 antibody (19-164) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat cerebellum using COX6A1 antibody (19-164) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using COX6A1 antibody (19-164) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using COX6A1 antibody (19-164) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using COX6A1 Polyclonal Antibody (19-164) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using COX6A1 Polyclonal Antibody (19-164) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using COX6A1 Polyclonal Antibody (19-164) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.