DDB1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-18-857
Artikelname: DDB1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-18-857
Hersteller Artikelnummer: 18-857
Alternativnummer: PRS-18-857-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: A synthetic peptide of human DDB1
Konjugation: Unconjugated
Alternative Synonym: DDB1, damage-specific DNA binding protein 1, 127 kDa, DDBA, XAP1, XPCE, XPE-BF, UV-DDB1, DDB p127 subunit, damage-specific DNA binding protein 1 (127kD)
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 127kDa
NCBI: 1642
UniProt: Q16531
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using DDB1 Antibody (18-857) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat testis using DDB1 Antibody (18-857) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using DDB1 Antibody (18-857) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using DDB1 Antibody (18-857) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using DDB1 Polyclonal Antibody (18-857) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using DDB1 Polyclonal Antibody (18-857) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.