IRF2 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-18-709
Artikelname: IRF2 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-18-709
Hersteller Artikelnummer: 18-709
Alternativnummer: PRS-18-709-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-349 of human IRF2 (NP_002190.2).
Konjugation: Unconjugated
Alternative Synonym: IRF2, IRF-2
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 42kDa
NCBI: 3660
UniProt: P14316
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using IRF2 antibody (18-709) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human breast cancer using IRF2 antibody (18-709) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using IRF2 antibody (18-709) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using IRF2 antibody (18-709) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using IRF2 antibody (18-709) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using IRF2 antibody (18-709). Blue: DAPI for nuclear staining.