mTOR Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
PRS-18-651
- Bilder (6)
| Artikelname: | mTOR Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | PRS-18-651 |
| Hersteller Artikelnummer: | 18-651 |
| Alternativnummer: | PRS-18-651-100 |
| Hersteller: | ProSci |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IP, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human mTOR (NP_004949.1). |
| Konjugation: | Unconjugated |
| Alternative Synonym: | TOR Antibody: FRAP, FRAP1, FRAP2, RAFT1, RAPT1, FRAP, FK506-binding protein 12-rapamycin complex-associated protein 1, mTOR |
| Application Verdünnung: | Optimal dilutions for each application to be determined by the researcher. |
| Anwendungsbeschreibung: | WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:100 |
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Western blot analysis of extracts of various cell lines, using mTOR antibody (18-651) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
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Immunofluorescence analysis of HeLa cells using mTOR antibody (18-651) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of PC12 cells using mTOR antibody (18-651) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of C6 cells using MTOR antibody (18-651) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of NIH/3T3 cells using MTOR antibody (18-651) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug mTOR antibody (18-651). Western blot was performed from the immunoprecipitate using mTOR antibody (18-651) at a dilition of 1:1000. |
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