DiMethyl-Histone H3-K79 pAb, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-18-631
Artikelname: DiMethyl-Histone H3-K79 pAb, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-18-631
Hersteller Artikelnummer: 18-631
Alternativnummer: PRS-18-631-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ChIP, IF, IHC, IP, WB
Spezies Reaktivität: Human, Mouse, Other, Rat
Immunogen: A synthetic peptide of human DiMethyl-Histone H3-K79
Konjugation: Unconjugated
Alternative Synonym: H3F3A, H3t, H3.4, H3/g, H3FT
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 17kDa
NCBI: 8290
UniProt: Q16695
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:1000IHC: 1:50 - 1:100IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:50 - 1:200CHIP seq: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K79 antibody (18-631).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K79 antibody (18-631).
Immunohistochemistry of paraffin-embedded human mammary cancer using DiMethyl-Histone H3-K79 antibody (18-631) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using DiMethyl-Histone H3-K79 antibody (18-631) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using DiMethyl-Histone H3-K79 antibody (18-631) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K79 antibody (18-631). Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation analysis of gamma-actin gene from 293 cell line, using DiMethyl-Histone H3-K79 antibody (18-631) and rabbit IgG. P1, P2 and P3 were probes located on gamma-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.