SFTPC Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-18-339
Artikelname: SFTPC Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-18-339
Hersteller Artikelnummer: 18-339
Alternativnummer: PRS-18-339-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-197 of human SFTPC (NP_001165881.1).
Konjugation: Unconjugated
Alternative Synonym: Pulmonary surfactant-associated protein C, SP-C, Pulmonary surfactant-associated proteolipid SPL(Val), SP5, SFTPC, SFTP2
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 21kDa
NCBI: 6440
UniProt: P11686
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using SFTPC antibody (18-339) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung using SFTPC antibody (18-339) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using SFTPC antibody (18-339) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using SFTPC antibody (18-339) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of human lung cancer using SFTPC Polyclonal Antibody (18-339) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse lung using SFTPC Polyclonal Antibody (18-339) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.