PTCH1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-15-720
Artikelname: PTCH1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-15-720
Hersteller Artikelnummer: 15-720
Alternativnummer: PRS-15-720-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 770-970 of human PTCH1 (NP_000255.2).
Konjugation: Unconjugated
Alternative Synonym: PTCH1, PTC, BCNS, HPE7, PTC1, PTCH, NBCCS, PTCH11
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 180kDa
NCBI: 5727
UniProt: Q13635
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IF: 1:50 - 1:200
Western blot analysis of extracts of rat skeletal muscle, using PTCH1 antibody (15-720) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunofluorescence analysis of C6 cells using PTCH1 antibody (15-720) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L-929 cells using PTCH1 antibody (15-720) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse embryos cells using PTCH1 antibody (15-720) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using PTCH1 antibody (15-720) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse embryos cells using PTCH1 antibody (15-720) at dilution of 1:100. Blue: DAPI for nuclear staining.