EP300 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-14-746
Artikelname: EP300 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-14-746
Hersteller Artikelnummer: 14-746
Alternativnummer: PRS-14-746-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EP300 (NP_001420.2).
Konjugation: Unconjugated
Alternative Synonym: EP300, E1A binding protein p300, Histone acetyltransferase p300, KAT3B, p300 HAT, E1A-associated protein p300, E1A-binding protein, 300kD, p300, RSTS2
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 280kDa
NCBI: 2033
UniProt: Q09472
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using EP300 antibody (14-746) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat ovary using EP300 antibody (14-746) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using EP300 antibody (14-746) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using EP300 antibody (14-746) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using EP300 antibody (14-746) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EP300 antibody (14-746) at dilution of 1:100 (40x lens).