Gm13125 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-14-546
Artikelname: Gm13125 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-14-546
Hersteller Artikelnummer: 14-546
Alternativnummer: PRS-14-546-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-477 of mouse Gm13125 (NP_001108549.1).
Konjugation: Unconjugated
Alternative Synonym: EG627009, Gm13125, novel preferentially expressed antigen in melanoma (Prame) protein
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 55kDa
NCBI: 627009
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using Gm13125 antibody (14-546) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using GM13125 antibody (14-546) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using GM13125 antibody (14-546) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using GM13125 antibody (14-546) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using GM13125 antibody (14-546) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using GM13125 antibody (14-546) at dilution of 1:100 (40x lens).