EIF2AK4 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-14-545
Artikelname: EIF2AK4 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-14-545
Hersteller Artikelnummer: 14-545
Alternativnummer: PRS-14-545-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EIF2AK4 (NP_001013725.2).
Konjugation: Unconjugated
Alternative Synonym: EIF2AK4, GCN2, GCN2 eIF2alpha kinase, GCN2-like protein, Mgcn2, KIAA1338
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 187kDa
NCBI: 440275
UniProt: Q9P2K8
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of HepG2 cells, using EIF2AK4 Antibody (14-545) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat testis using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using EIF2AK4 antibody (14-545) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EIF2AK4 antibody (14-545) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using EIF2AK4 antibody (14-545). Blue: DAPI for nuclear staining.