Western blot analysis of extracts of various cell lines, using PRPF19 antibody (14-527) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat liver using PRPF19 antibody (14-527) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using PRPF19 antibody (14-527) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PRPF19 antibody (14-527) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PRPF19 antibody (14-527) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug PRPF19 antibody (14-527). Western blot was performed from the immunoprecipitate using PRPF19 antibody (14-527) at a dilition of 1:1000.
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