CD8A Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: PRS-14-172
Artikelname: CD8A Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: PRS-14-172
Hersteller Artikelnummer: 14-172
Alternativnummer: PRS-14-172-100
Hersteller: ProSci
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-182 of human CD8A (NP_001759.3).
Konjugation: Unconjugated
Alternative Synonym: T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a, CD8A, MAL
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 30kDa
NCBI: 925
UniProt: P01732
Puffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Formulierung: Liquid
Application Verdünnung: Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of mouse pancreas, using CD8A antibody (14-172) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human tonsil using CD8A antibody (14-172) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using CD8A antibody (14-172) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CD8A antibody (14-172) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using CD8A antibody (14-172) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using CD8A antibody (14-172) at dilution of 1:100. Blue: DAPI for nuclear staining.