FAK Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
PRS-13-952
- Bilder (7)
| Artikelname: | FAK Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | PRS-13-952 |
| Hersteller Artikelnummer: | 13-952 |
| Alternativnummer: | PRS-13-952-100 |
| Hersteller: | ProSci |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC, IP, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 700-800 of human FAK (NP_722560.1). |
| Konjugation: | Unconjugated |
| Alternative Synonym: | PTK2, FADK, Fadk1, Focal adhesion kinase, FRNK, FADK 1, Focal adhesion kinase 1, FAK1, p125FAK, PPP1R71, Protein-tyrosine kinase 2, PTK2 protein tyrosine kinase 2, FAK, pp125 FA kinase, Pp125FAK |
| Application Verdünnung: | Optimal dilutions for each application to be determined by the researcher. |
| Anwendungsbeschreibung: | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100 |
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Western blot analysis of extracts of various cell lines, using PTK2 antibody (13-952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. |
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Immunohistochemistry of paraffin-embedded human colon using FAK antibody (13-952) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human stomach using FAK antibody (13-952) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of HeLa cells using FAK antibody (13-952) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of PC12 cells using FAK antibody (13-952) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of RAW264.7 cells using FAK antibody (13-952) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug FAK antibody (13-952). Western blot was performed from the immunoprecipitate using FAK antibody (13-952) at a dilition of 1:1000. |
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