Optimal dilutions for each application to be determined by the researcher.
Anwendungsbeschreibung:
WB: 1:500 - 1:1000IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using PTCH1 antibody (13-291) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of C6 cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse embryos cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse embryos cells using PTCH1 antibody (13-291) at dilution of 1:100. Blue: DAPI for nuclear staining.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten