HDAC1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
PRS-13-098
- Bilder (6)
| Artikelname: | HDAC1 Antibody, KO Validated, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | PRS-13-098 |
| Hersteller Artikelnummer: | 13-098 |
| Alternativnummer: | PRS-13-098-100 |
| Hersteller: | ProSci |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC, IP, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (NP_004955.2). |
| Konjugation: | Unconjugated |
| Alternative Synonym: | HDAC1 Antibody: HD1, RPD3, GON-10, RPD3L1, Histone deacetylase 1, HD1 |
| Application Verdünnung: | Optimal dilutions for each application to be determined by the researcher. |
| Anwendungsbeschreibung: | WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:100 |
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Western blot analysis of extracts of various cell lines, using HDAC1 antibody (13-098) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. |
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Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody (13-098) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s. |
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Immunohistochemistry of paraffin-embedded human prostate cancer using HDAC1 antibody (13-098) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human stomach using HDAC1 antibody (13-098) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of A549 cells using HDAC1 antibody (13-098). Blue: DAPI for nuclear staining. |
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Immunoprecipitation analysis of 200ug extracts of HT-29 cells, using 3 ug HDAC1 antibody (13-098). Western blot was performed from the immunoprecipitate using HDAC1 antibody (13-098) at a dilition of 1:1000. |
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