Biotinylated sheep immunoglobulin was used as the immunogen for the Biotin antibody.
This mAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. It is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[BTN/8672R]
UniProt:
Not Applicable
Reinheit:
Protein A affinity
Formulierung:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Antibody Type:
Primary Antibody
Application Verdünnung:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Anwendungsbeschreibung:
Optimal dilution of the Biotin antibody should be determined by the researcher.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
