gp96 Antibody / GRP94 / HSP90B1, Clone: [SPM249], Rat, Monoclonal
Artikelnummer:
NSJ-V2909SAF-100UG
Hersteller Artikelnummer:
V2909SAF-100UG
Alternativnummer:
NSJ-V2909SAF-100UG
Hersteller:
NSJ Bioreagents
Wirt:
Rat
Kategorie:
Antikörper
Applikation:
FACS, IF, IHC-P
Spezies Reaktivität:
Human
Immunogen:
Purified glucose regulated protein 94 from chicken oviducts was used as the immunogen for the gp96 antibody.
Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Gp96 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This mAb is highly specific and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Gp96 has been briefly studied as a prognostic factor in breast cancer.
Flow cytometry: 1-2ug/10 6 cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Anwendungsbeschreibung:
Optimal dilution of the gp96 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
