Laminin gamma 1 Antibody / LAMC1 / LAMB2, Rat, Monoclonal
Artikelnummer:
NSJ-V2682SAF-100UG
Hersteller Artikelnummer:
V2682SAF-100UG
Alternativnummer:
NSJ-V2682SAF-100UG
Hersteller:
NSJ Bioreagents
Wirt:
Rat
Kategorie:
Antikörper
Applikation:
FACS, IF, IHC-P
Spezies Reaktivität:
Human, Mouse
Immunogen:
A murine EHS laminin preparation was used as the immunogen for the Laminin gamma 1 antibody.
Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This mAb reacts with laminin B2/1 chain of ~210kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific mAbs. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This mAb stains uniformly all human and murine basement membranes.
Flow cytometry: 0.5-1ug/10 6 cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Anwendungsbeschreibung:
Optimal dilution of the Laminin gamma 1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
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