A portion of amino acids 212-239 from the human protein were used as the immunogen for the ADH5 antibody.
ADH5 is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis.
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Anwendungsbeschreibung:
The stated application concentrations are suggested starting points. Titration of the ADH5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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