A portion of amino acids 161-190 from the human protein were used as the immunogen for the ATG16L1 Antibody.
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The APG12-APG5-APG16L complex is esential for the elongation of autophagic isolation membranes. This complex initially associates in uniform distribution with small vesicle membranes. During membrane elongation, the complex partitions, with a great concentration building on the outer side of the isolation membrane. Upon completion of the formation of the autophagosome, the APG12-APG5-APG16L dissociates from the membrane.
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25
Anwendungsbeschreibung:
The stated application concentrations are suggested starting points. Titration of the ATG16L1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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