Amino acids surrounding phosphorylated serine 668 from human MYPT1 were used as the immunogen for the phospho-MYPT1 antibody.
Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN- dependent suppression of HIF1A activity. [UniProt]
The stated application concentrations are suggested starting points. Titration of the phospho-MYPT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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