A synthetic SOD2-specific peptide was used as the immunogen for the SOD2 antibody.
Superoxide dismutase 2 is a member of the iron/manganese superoxide dismutase family. The SOD2 gene encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. [Wiki]
The stated application concentrations are suggested starting points. Titration of the SOD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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