A portion of amino acids 271-300 from the human protein was used as the immunogen for this Cdc25A antibody.
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. [UniProt]
The stated application concentrations are suggested starting amounts. Titration of the Cdc25A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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