Mobicol Spin Ni-IDA

Ni-IDA Columns for Purification of His-tagged Proteins.

Features

• Easy, fast, and cost-effective routine purification of recombinant polyhistidine-tagged proteins under native and denaturing conditions starting from diverse expression systems, e.g., E. coli, yeast, insect, and mammalian cells
• Universal use: suitable for small proteins, large protein complexes, proteins with low expression rates
• High binding capacity and high affinity
• Improved target specificity by optimized silica-based Ni2+-IDA matrix
• Imidazol-free loading and washing buffer.

His-Tagged Protein Purification

Histidine-tagged protein purification uses affinity chromatography to capture recombinant proteins with 4 - 10 histidine residues. Ni-IDA columns from MoBiTec contain a silica-based resin. The form-stable silica matrix is pre-charged with Ni2+ ions and allows purification on the principle of Immobilized Metal Ion Affinity Chromatography (IMAC). Binding of proteins is based on the interaction between the polyhistidine tag of a recombinant protein and immobilized Ni2+ ions. The chelating group of the Ni-IDA resin is based on iminodiacetic acid (IDA), which enables strong and efficient binding of target protein to the IMAC matrix. In contrast to traditional IDA matrices, MoBiTec Ni-IDA matrix is an optimized matrix with low density of IDA ligands. This non-saturating surface concentration of IDA eliminates almost all non-specific interactions of contaminating host proteins with the adsorbent. As a result, Lab Columns Ni-IDA and Mobicol Spin Ni-IDA columns provide much higher target protein purity.

Advantages of Mobicol Spin Ni-IDA Columns:

• Pre-packed, single-use spin columns for simple, small-scale purification of histidine-tagged proteins and rapid expression screening.
• For protein purification by spinning
• Compatible with standard microcentrifuges
• The purification run takes approximately 10 min
• Very high binding capacity: up to 12 mg protein per spin column
• Excellent protein recovery rate of > 90%
• No commercial license required