Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)]. Not yet tested in other applications. Optima
Sample (30 ug of whole cell lysate) A: SK-N-SH 7.5% SDS PAGE SH2D3C antibody diluted at 1:500
IHC of paraffin-embedded Hepatoma, using SH2D3C antibody at 1:500 dilution.
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