Protein A (MW 42 kDa) is capable of binding to the Fc portion of immunoglobulins, especially IgGs, from a large number of species. Phycoethyrin (R-PE) is a 240 kDa protein from the light-harvesting phycobilliprotein, phycoerythrin is composed of a protein part, organised in a hexameric structure of alpha and beta chains, covalently binding chromophores called phycobilins.
Puffer:
10 mM PO4, 150 mM NaCl, 1% BSA and 0.05% sodium azide
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