TMEM176B Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA023758LA01HU
- Bilder (5)
| Artikelname: | TMEM176B Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA023758LA01HU |
| Hersteller Artikelnummer: | CSB-PA023758LA01HU |
| Alternativnummer: | CSB-PA023758LA01HU-100UG, CSB-PA023758LA01HU-50UG |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | TMEM176B antibody, LR8 antibody, Transmembrane protein 176B antibody, Protein LR8 antibody |
| Klonalität: | Polyclonal |
| UniProt: | Q3YBM2 |
| Puffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Reinheit: | Antigen Affinity Purified |
| Formulierung: | Liquid |
| Target-Kategorie: | TMEM176B |
| Application Verdünnung: | Recommended dilution: WB:1:500-1:2000, IF:1:10-1:100 |
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Immunohistochemistry of paraf |
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Immunofluorescence staining of Hela cell with CSB-PA023758LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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Western Blot Positive WB detected in: THP-1 whole cell lysate(30µg), SY5Y whole cell lysate(30µg), Hela whole cell lysate(30µg), K562 whole cell lysate(30µg) All lanes: TMEM176B antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 30,26 kDa Observed band size: 30 kDa Exposure time:120s |
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