Immunofluorescent analysis of Hela cells using CSB-PA01459A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Western Blot Positive WB detected in: Raji whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, Mouse spleen tissue, Mouse lung tissue All lanes: YWHAZ antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 28, 20 kDa Observed band size: 28, 70 kDa
IHC image of CSB-PA01459A0Rb diluted at 1:1000 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA01459A0Rb diluted at 1:1000 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA01459A0Rb diluted at 1:1000 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten