HIST1H4A (Ab-12) Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA010429OA12NBUTHU
- Bilder (5)
| Artikelname: | HIST1H4A (Ab-12) Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA010429OA12NBUTHU |
| Hersteller Artikelnummer: | CSB-PA010429OA12nbutHU |
| Alternativnummer: | CSB-PA010429OA12NBUTHU-100UL, CSB-PA010429OA12NBUTHU-50UL |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ChIP, ELISA, IHC, IP, WB |
| Spezies Reaktivität: | Human, Mouse |
| Konjugation: | Unconjugated |
| Alternative Synonym: | dJ160A22.1 antibody, dJ160A22.2 antibody, dJ221C16.1 antibody, dJ221C16.9 antibody, FO108 antibody, H4 antibody, H4.k antibody, H4/a antibody, H4/b antibody, H4/c antibody, H4/d antibody, H4/e antibody, H4/g antibody, H4/h antibody, H4/I antibody, H4/j antibody, H4/k antibody, H4/m antibody, H4/n antibody, H4/p antibody, H4_HUMAN antibody, H4F2 antibody, H4F2iii antibody, H4F2iv antibody, H4FA antibody, H4FB antibody, H4FC antibody, H4FD antibody, H4FE antibody, H4FG antibody, H4FH antibody, H4FI antibody, H4FJ antibody, H4FK antibody, H4FM antibody, H4FN antibody, H4M antibody, HIST1H4A antibody, HIST1H4B antibody, HIST1H4C antibody, HIST1H4D antibody, HIST1H4E antibody, HIST1H4F antibody, HIST1H4H antibody, HIST1H4I antibody, HIST1H4J antibody, HIST1H4K antibody, HIST1H4L antibody, HIST2H4 antibody, HIST2H4A antibody, Hist4h4 antibody, Histone 1 H4a antibody, Histone 1 H4b antibody, Histone 1 H4c antibody, Histone 1 H4d antibody, Histone 1 H4e antibody, Histone 1 H4f antibody, Histone 1 H4h antibody, Histone 1 H4i antibody, Histone 1 H4j antibody, Histone 1 H4k antibody, Histone 1 H4l antibody, Histone 2 H4a antibody, histone 4 H4 antibody, Histone H4 antibody, MGC24116 antibody |
| Klonalität: | Polyclonal |
| UniProt: | P62805 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Reinheit: | Antigen Affinity Purified |
| Formulierung: | Liquid |
| Target-Kategorie: | HIST1H4A |
| Application Verdünnung: | Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IP:1:200-1:2000 |
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Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H4A (CSB-PA010429OA12nbutHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter. |
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IHC image of CSB-PA010429OA12nbutHU diluted at 1:100 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-PA010429OA12nbutHU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Immunoprecipitating HIST1H4A in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA010429OA12nbutHU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA010429OA12nbutHU (3µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg) |
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Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate All lanes: HIST1H4A antibody at 0.16µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 12 kDa Observed band size: 12 kDa |
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