HIST1H2BC (Ab-23) Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA010403OA23NCRHU
- Bilder (4)
| Artikelname: | HIST1H2BC (Ab-23) Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA010403OA23NCRHU |
| Hersteller Artikelnummer: | CSB-PA010403OA23ncrHU |
| Alternativnummer: | CSB-PA010403OA23NCRHU-100UL, CSB-PA010403OA23NCRHU-50UL |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | H2BC4 antibody, H2BFL antibody, HIST1H2BC, antibody, H2BC6 antibody, H2BFH antibody, HIST1H2BE, antibody, H2BC7 antibody, H2BFG antibody, HIST1H2BF, antibody, H2BC8 antibody, H2BFA antibody, HIST1H2BG, antibody, H2BC10 antibody, H2BFK antibody, HIST1H2BIHistone H2B type 1-C/E/F/G/I antibody, Histone H2B.1 A antibody, Histone H2B.a antibody, H2B/a antibody, Histone H2B.g antibody, H2B/g antibody, Histone H2B.h antibody, H2B/h antibody, Histone H2B.k antibody, H2B/k antibody, Histone H2B.l antibody, H2B/l antibody |
| Klonalität: | Polyclonal |
| UniProt: | P62807 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Reinheit: | Antigen Affinity Purified |
| Formulierung: | Liquid |
| Target-Kategorie: | HIST1H2BC |
| Application Verdünnung: | Recommended dilution: WB:1:100-1:1000, IHC:1:10-1:100, IF:1:1-1:10 |
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Immunofluorescence staining of Hela cells with CSB-PA010403OA23ncrHU at 1:1, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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IHC image of CSB-PA010403OA23ncrHU diluted at 1:10 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-PA010403OA23ncrHU diluted at 1:10 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: HIST1H2BC antibody at 0.04µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa |
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