Crotonyl-HIST1H2BC (K23) Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA010403OA23CRHU
- Bilder (4)
| Artikelname: | Crotonyl-HIST1H2BC (K23) Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA010403OA23CRHU |
| Hersteller Artikelnummer: | CSB-PA010403OA23crHU |
| Alternativnummer: | CSB-PA010403OA23CRHU-100UL, CSB-PA010403OA23CRHU-50UL |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ChIP, ELISA, IF, IP, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | H2BC4 antibody, H2BFL antibody, HIST1H2BC, antibody, H2BC6 antibody, H2BFH antibody, HIST1H2BE, antibody, H2BC7 antibody, H2BFG antibody, HIST1H2BF, antibody, H2BC8 antibody, H2BFA antibody, HIST1H2BG, antibody, H2BC10 antibody, H2BFK antibody, HIST1H2BIHistone H2B type 1-C/E/F/G/I antibody, Histone H2B.1 A antibody, Histone H2B.a antibody, H2B/a antibody, Histone H2B.g antibody, H2B/g antibody, Histone H2B.h antibody, H2B/h antibody, Histone H2B.k antibody, H2B/k antibody, Histone H2B.l antibody, H2B/l antibody |
| Klonalität: | Polyclonal |
| UniProt: | P62807 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Reinheit: | Antigen Affinity Purified |
| Formulierung: | Liquid |
| Target-Kategorie: | HIST1H2BC |
| Application Verdünnung: | Recommended dilution: WB:1:100-1:1000, IF:1:20-1:200, IP:1:200-1:2000 |
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Immunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) with CSB-PA010403OA23crHU at 1:37.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (CSB-PA010403OA23crHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter. |
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Immunoprecipitating HIST1H2BC in K562 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA010403OA23crHU in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA010403OA23crHU (3µg) + K562 whole cell lysate (500µg) Lane 3: K562 whole cell lysate (20µg) |
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Western Blot Positive WB detected in: Hela whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate All lanes: HIST1H2BC antibody at 1.8µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa |
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