Butyrly-HIST1H2BC (K5) Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA010403OA05BUTHU
- Bilder (4)
| Artikelname: | Butyrly-HIST1H2BC (K5) Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA010403OA05BUTHU |
| Hersteller Artikelnummer: | CSB-PA010403OA05butHU |
| Alternativnummer: | CSB-PA010403OA05BUTHU-100UL, CSB-PA010403OA05BUTHU-50UL |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ChIP, ELISA, IF, IP, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | H2BC4 antibody, H2BFL antibody, HIST1H2BC, antibody, H2BC6 antibody, H2BFH antibody, HIST1H2BE, antibody, H2BC7 antibody, H2BFG antibody, HIST1H2BF, antibody, H2BC8 antibody, H2BFA antibody, HIST1H2BG, antibody, H2BC10 antibody, H2BFK antibody, HIST1H2BIHistone H2B type 1-C/E/F/G/I antibody, Histone H2B.1 A antibody, Histone H2B.a antibody, H2B/a antibody, Histone H2B.g antibody, H2B/g antibody, Histone H2B.h antibody, H2B/h antibody, Histone H2B.k antibody, H2B/k antibody, Histone H2B.l antibody, H2B/l antibody |
| Klonalität: | Polyclonal |
| UniProt: | P62807 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Reinheit: | Antigen Affinity Purified |
| Formulierung: | Liquid |
| Target-Kategorie: | HIST1H2BC |
| Application Verdünnung: | Recommended dilution: WB:1:100-1:1000, IF:1:10-1:100, IP:1:200-1:2000 |
|
|
Western Blot Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Untreated (-) or treated (+) with 30mM sodium butyrate for 4h All lanes: HIST1H2BC antibody at 1:100 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa |
|
|
Chromatin Immunoprecipitation Hela (106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (CSB-PA010403OA05butHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter. |
|
|
Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010403OA05butHU at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
|
|
Immunoprecipitating HIST1H2BC in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA010403OA05butHU in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA010403OA05butHU (5µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (20µg) |
Produktgarantie und fachkundiger Support
