Acetyl-HIST1H2AG (K13) Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA010389OA13ACHU
- Bilder (4)
| Artikelname: | Acetyl-HIST1H2AG (K13) Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA010389OA13ACHU |
| Hersteller Artikelnummer: | CSB-PA010389OA13acHU |
| Alternativnummer: | CSB-PA010389OA13ACHU-100UL, CSB-PA010389OA13ACHU-50UL |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC, IP, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | H2AC11 antibody, H2AFP antibody, HIST1H2AG, antibody, H2AC13 antibody, H2AFC antibody, HIST1H2AI, antibody, H2AC15 antibody, H2AFD antibody, HIST1H2AK, antibody, H2AC16 antibody, H2AFI antibody, HIST1H2AL, antibody, H2AC17 antibody, H2AFN antibody, HIST1H2AMHistone H2A type 1 antibody, H2A.1 antibody, Histone H2A/ptl antibody |
| Klonalität: | Polyclonal |
| UniProt: | P0C0S8 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Reinheit: | Antigen Affinity Purified |
| Formulierung: | Liquid |
| Target-Kategorie: | HIST1H2AG |
| Application Verdünnung: | Recommended dilution: WB:1:100-1:1000, IHC:1:1-1:10, IF:1:1-1:10, IP:1:200-1:2000 |
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Immunocytochemistry analysis of CSB-PA010389OA13acHU diluted at 1:5 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010389OA13acHU at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate (all treated with 30mM sodium butyrate for 4h) All lanes: HIST1H2AG antibody at 1.25µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa |
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Immunoprecipitating HIST1H2AG in 293 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA010389OA13acHU in 293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA010389OA13acHU (5µg) + 293 whole cell lysate (500µg) Lane 3: 293 whole cell lysate (20µg) |
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