HIST1H1E (Ab-25) Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: CSB-PA010380PA25NME1HU
Artikelname: HIST1H1E (Ab-25) Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: CSB-PA010380PA25NME1HU
Hersteller Artikelnummer: CSB-PA010380PA25nme1HU
Alternativnummer: CSB-PA010380PA25NME1HU-100UL, CSB-PA010380PA25NME1HU-50UL
Hersteller: Cusabio
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ChIP, ELISA, IF, IHC, WB
Spezies Reaktivität: Human
Konjugation: Unconjugated
Alternative Synonym: H1 histone family member 4 antibody, H1.4 antibody, H14_HUMAN antibody, H1E antibody, H1F4 antibody, Hist1h1e antibody, Histone 1 H1e antibody, Histone cluster 1 H1e antibody, Histone H1 antibody, Histone H1.4 antibody, Histone H1B antibody, MGC116819 antibody
Klonalität: Polyclonal
UniProt: P10412
Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Reinheit: Antigen Affinity Purified
Formulierung: Liquid
Target-Kategorie: HIST1H1E
Application Verdünnung: Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200
Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H1E (CSB-PA010380PA25nme1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.
Immunofluorescent analysis of Hela cells using CSB-PA010380PA25nme1HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA010380PA25nme1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA010380PA25nme1HU at dilution of 1:100
Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, LO2 whole cell lysate
All lanes: HIST1H1E antibody at 1:500
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa