HIST1H1E (Ab-45) Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA010380OA45NACHU
- Bilder (4)
| Artikelname: | HIST1H1E (Ab-45) Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA010380OA45NACHU |
| Hersteller Artikelnummer: | CSB-PA010380OA45nacHU |
| Alternativnummer: | CSB-PA010380OA45NACHU-100UL, CSB-PA010380OA45NACHU-50UL |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | H1 histone family member 4 antibody, H1.4 antibody, H14_HUMAN antibody, H1E antibody, H1F4 antibody, Hist1h1e antibody, Histone 1 H1e antibody, Histone cluster 1 H1e antibody, Histone H1 antibody, Histone H1.4 antibody, Histone H1B antibody, MGC116819 antibody |
| Klonalität: | Polyclonal |
| UniProt: | P10412 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Reinheit: | Antigen Affinity Purified |
| Formulierung: | Liquid |
| Target-Kategorie: | HIST1H1E |
| Application Verdünnung: | Recommended dilution: WB:1:100-1:1000, IHC:1:1-1:10, IF:1:1-1:10 |
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Immunofluorescence staining of Hela cells with CSB-PA010380OA45nacHU at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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IHC image of CSB-PA010380OA45nacHU diluted at 1:5 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-PA010380OA45nacHU diluted at 1:5 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Western Blot Positive WB detected in: 293 whole cell lysate All lanes: HIST1H1E antibody at 0.94µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa |
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