HIST1H1C (Ab-96) Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA010378OA96NFORHU
- Bilder (4)
| Artikelname: | HIST1H1C (Ab-96) Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA010378OA96NFORHU |
| Hersteller Artikelnummer: | CSB-PA010378OA96nforHU |
| Alternativnummer: | CSB-PA010378OA96NFORHU-100UL, CSB-PA010378OA96NFORHU-50UL |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | H1 histone family member 2 antibody, H1.a antibody, H12_HUMAN antibody, H1F2 antibody, H1s-1 antibody, HIST1H1C antibody, Histone 1 H1c antibody, Histone cluster 1 H1c antibody, Histone H1.2 antibody, Histone H1c antibody, Histone H1d antibody, Histone H1s-1 antibody, MGC3992 antibody |
| Klonalität: | Polyclonal |
| UniProt: | P16403 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Reinheit: | Antigen Affinity Purified |
| Formulierung: | Liquid |
| Target-Kategorie: | HIST1H1C |
| Application Verdünnung: | Recommended dilution: WB:1:100-1:1000, IHC:1:20-1:200, IF:1:1-1:10 |
|
|
Immunofluorescence staining of Hela cells with CSB-PA010378OA96nforHU at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
|
|
IHC image of CSB-PA010378OA96nforHU diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
|
|
IHC image of CSB-PA010378OA96nforHU diluted at 1:50 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
|
|
Western Blot Positive WB detected in: MCF-7 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate All lanes: HIST1H1C antibody at 1.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa |
Produktgarantie und fachkundiger Support
