Di-methyl-HIST1H1C (K45) Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA010378OA45ME2HU
- Bilder (4)
| Artikelname: | Di-methyl-HIST1H1C (K45) Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA010378OA45ME2HU |
| Hersteller Artikelnummer: | CSB-PA010378OA45me2HU |
| Alternativnummer: | CSB-PA010378OA45ME2HU-100UL, CSB-PA010378OA45ME2HU-50UL |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ChIP, ELISA, ICC, IP, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | H1 histone family member 2 antibody, H1.a antibody, H12_HUMAN antibody, H1F2 antibody, H1s-1 antibody, HIST1H1C antibody, Histone 1 H1c antibody, Histone cluster 1 H1c antibody, Histone H1.2 antibody, Histone H1c antibody, Histone H1d antibody, Histone H1s-1 antibody, MGC3992 antibody |
| Klonalität: | Polyclonal |
| UniProt: | P16403 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Reinheit: | Antigen Affinity Purified |
| Formulierung: | Liquid |
| Target-Kategorie: | HIST1H1C |
| Application Verdünnung: | Recommended dilution: WB:1:1000-1:5000, ICC:1:10-1:100, IP:1:200-1:2000 |
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Western Blot Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate All lanes: HIST1H1C antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa |
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Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H1C (CSB-PA010378OA45me2HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter. |
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Immunocytochemistry analysis of CSB-PA010378OA45me2HU diluted at 1:10 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Immunoprecipitating HIST1H1C in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA010378OA45me2HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA010378OA45me2HU (5µg) + Hela whole cell lysate (1mg) Lane 3: Hela whole cell lysate (20µg) |
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