Chromatin Immunoprecipitation Hela(1.2*106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-HDAC3 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers(CSB-PP010239HU) against the P21 promoter.
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA010239LA01HU at dilution of 1:100
Western blot All lanes: HDAC3 antibody at 2µg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49, 50 kDa Observed band size: 49 kDa
Western Blot Positive WB detected in: Mouse heart tissue All lanes: HDAC3 antibody at 2.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 49, 50 kDa Observed band size: 49 kDa
Immunofluorescent analysis of Hela cells using CSB-PA010239LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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