GPR32 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA009803LA01HU
- Bilder (4)
| Artikelname: | GPR32 Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA009803LA01HU |
| Hersteller Artikelnummer: | CSB-PA009803LA01HU |
| Alternativnummer: | CSB-PA009803LA01HU-100UG, CSB-PA009803LA01HU-50UG |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | G protein coupled receptor GPR32 antibody, GPR32 antibody, GPR32_HUMAN antibody, Probable G protein coupled receptor 32 antibody, Probable G-protein coupled receptor 32 antibody |
| Klonalität: | Polyclonal |
| UniProt: | O75388 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Reinheit: | >95%, Protein G purified |
| Formulierung: | Liquid |
| Target-Kategorie: | GPR32 |
| Application Verdünnung: | Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:50-1:200 |
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Immunofluorescence staining of A549 cells with CSB-PA009803LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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IHC image of CSB-PA009803LA01HU diluted at 1:500 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-PA009803LA01HU diluted at 1:500 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Western Blot Positive WB detected in: HL60 whole cell lysate All lanes: GPR32 antibody at 2.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa |
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